human leukemic jurkat e6.1 cell line Search Results


human leukemic jurkat e6.1 cell line  (European Collection of Authenticated Cell Cultures)
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European Collection of Authenticated Cell Cultures human leukemic jurkat e6.1 cell line
Proliferation rates of the T-cell lines and effects of cladribine on cell viability. (a) The growth rate of <t>Jurkat</t> E6.1 is faster than the CCRF-CEM cell line ( n = 3). (b) A cladribine dose response curve in Jurkat E6.1 cell line for cell proliferation with 48 hours ( n = 5) and 96 hours culture ( n = 5). (c) The effect of cladribine on cell proliferation in Jurkat with 12 days ( n = 5) and 16 days in culture ( n = 5). (d) The effect of cladribine on cell proliferation in CCRF-CEM with 2 days ( n = 5), 8 days ( n = 4), and 12 days ( n = 4) in culture ( n = 5).
Human Leukemic Jurkat E6.1 Cell Line, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proliferation rates of the T-cell lines and effects of cladribine on cell viability. (a) The growth rate of Jurkat E6.1 is faster than the CCRF-CEM cell line ( n = 3). (b) A cladribine dose response curve in Jurkat E6.1 cell line for cell proliferation with 48 hours ( n = 5) and 96 hours culture ( n = 5). (c) The effect of cladribine on cell proliferation in Jurkat with 12 days ( n = 5) and 16 days in culture ( n = 5). (d) The effect of cladribine on cell proliferation in CCRF-CEM with 2 days ( n = 5), 8 days ( n = 4), and 12 days ( n = 4) in culture ( n = 5).

Journal: Clinical and Experimental Immunology

Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine

doi: 10.1093/cei/uxad112

Figure Lengend Snippet: Proliferation rates of the T-cell lines and effects of cladribine on cell viability. (a) The growth rate of Jurkat E6.1 is faster than the CCRF-CEM cell line ( n = 3). (b) A cladribine dose response curve in Jurkat E6.1 cell line for cell proliferation with 48 hours ( n = 5) and 96 hours culture ( n = 5). (c) The effect of cladribine on cell proliferation in Jurkat with 12 days ( n = 5) and 16 days in culture ( n = 5). (d) The effect of cladribine on cell proliferation in CCRF-CEM with 2 days ( n = 5), 8 days ( n = 4), and 12 days ( n = 4) in culture ( n = 5).

Article Snippet: The human leukemic Jurkat E6.1 cell line (pseudodiploid, modal No 46) and the CCRF-CEM (ECACC 85112105) cell line (2 n = 46) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) general cell collection and cultured according to the recommended instructions (Jurkat E6.1: RPMI 1640; 2 mM glutamine; 10% FBS) (CCRF-CEM: RPMI 1640; 2 mM glutamine; 20% FBS).

Techniques:

Effect of cladribine on mitochondrial protein synthesis. (a) Immunoblot of MT-ATP6, MT-CO1, MRPL11, MRPS35, CASPASE3, SDHA, and CANX after 12 days culture with cladribine. In this experiment, 10 nM cladribine in CCRF cells resulted in extensive cell death, and immunoblot was not performed. (b) A pulse metabolic labeling of mitochondrial protein synthesis. The effect on mitochondrial protein synthesis in the CCRF-CEM and Jurkat cells after treatment with cladribine for 8 days ( n = 3) or 12 days ( n = 3).

Journal: Clinical and Experimental Immunology

Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine

doi: 10.1093/cei/uxad112

Figure Lengend Snippet: Effect of cladribine on mitochondrial protein synthesis. (a) Immunoblot of MT-ATP6, MT-CO1, MRPL11, MRPS35, CASPASE3, SDHA, and CANX after 12 days culture with cladribine. In this experiment, 10 nM cladribine in CCRF cells resulted in extensive cell death, and immunoblot was not performed. (b) A pulse metabolic labeling of mitochondrial protein synthesis. The effect on mitochondrial protein synthesis in the CCRF-CEM and Jurkat cells after treatment with cladribine for 8 days ( n = 3) or 12 days ( n = 3).

Article Snippet: The human leukemic Jurkat E6.1 cell line (pseudodiploid, modal No 46) and the CCRF-CEM (ECACC 85112105) cell line (2 n = 46) were obtained from the European Collection of Authenticated Cell Cultures (ECACC) general cell collection and cultured according to the recommended instructions (Jurkat E6.1: RPMI 1640; 2 mM glutamine; 10% FBS) (CCRF-CEM: RPMI 1640; 2 mM glutamine; 20% FBS).

Techniques: Western Blot, Labeling